France could welcome back captain Patrick Vieira after he missed their opening two games with a thigh problem.
Defender William Gallas missed training on Saturday but is expected to play for France, who must win to progress.
Italy coach Roberto Donadoni made five changes before their last game against Romania and also altered the formation following a thumping loss to the Dutch.
It resulted in a better performance from the world champions, who will choose from a fully fit squad.
Italy were eliminated from the group stages of Euro 2004 despite beating Bulgaria in their final game.
At the same time Denmark and Sweden drew 2-2 to both go through to the knockout stages.
Donadoni's side could scrape go through with a draw against France, but they are be relying on the Dutch beating Romania.
A score draw would see Italy progress with a Netherland win, while a 0-0 result would see the Azzurri requiring Romania to lose by at least 3-0.
The mathematics are easier for France, who know that they need to win to have a hope of making it to the quarter-finals.
One thing is for certain: at least one of the 2006 World Cup finalists will be heading home from the tournament on Wednesday.
"Nobody would have thought that Italy-France would be a last-ditch match," Italy goalkeeper Gianluigi Buffon said.
"Before the European Championship, this was supposed to be the biggest match of the group."
BIG MATCH STATS
France and Italy have met on 35 previous occasions. Italy have won 17 of these encounters, France only eight. One of these matches was the Euro 2000 final which was won by France (2-1). David Trezeguet scored the golden goal. Lilian Thuram, Patrick Vieira, Thierry Henry and Alessandro Del Piero played in that match.
These teams also contested the 2006 World Cup final. After drawing 1-1, Italy won the penalty shoot-out.
Two months later, France and Italy met again, this time in qualifying for Euro 2008. France won 3-1 courtesy of goals by Sidney Govou (two) and Thierry Henry. The return, a year later, ended in a 0-0 draw. That match was refereed by Lubos Michel, who has also been appointed for this clash.
Head to head records and significant meetings
Team facts - France
Can only qualify for the quarter-finals, if they beat Italy and if Romania fail to beat the Netherlands.
Fielded their oldest ever starting line-up in European Championship history in their previous match against the Netherlands (30 years and 133 days).
Team facts - Italy
Can qualify for the quarter-finals, if they defeat France and if Romania fail to beat the Netherlands. A draw involving goals (1-1, 2-2, etc.) can also be enough for Italy, providing Romania loses to the Netherlands. A 0-0 draw would also be enough, if the Netherlands beat Romania 3-0 or by at least a four goal margin.
Can become the first country at a European Championship to progress from a group without winning a single match.
Been knocked out in the group phase in two of the last three European Championships. The last two reigning world champions from Europe went on to at least reach the final of the next European Championship. Germany finished runners-up in 1992 and France won Euro 2000.
Italy and Turkey have already used 18 players after two matches in the tournament.
Player facts - France
By scoring against the Netherlands, Thierry Henry became the third player to score in three different European Championships, joining Jurgen Klinsmann (Germany) and Vladimir Smicer (Czech Republic).
Henry is also only the fourth player to score in at least five major tournaments, European Championship and World Cup combined, joining Jurgen Klinsmann, Rudi Voller and Henrik Larsson. Klinsmann holds the record with six.
Willy Sagnol, Jeremy Toulalan and Claude Makelele will be suspended for a possible quarter-final match if they receive a yellow card against France.
Player facts - Italy
If selected, Alessandro Del Piero will play his 13th European Championship match, equalling Italian record holder Paolo Maldini.
If Del Piero scores, he will become the first Italian to score in four different major tournaments (European Championships and World Cups).
Italy players to have scored in three major tournaments in world football
35-year-old Christian Panucci became the second oldest player to score at a European Championship when he netted against Romania.
A yellow card for Gianluca Zambrotta will make him the first player ever to receive six yellow cards at the European Championship.
Zambrotta, Luca Toni, Gennaro Gattuso, Andrea Pirlo and Daniele De Rossi will be suspended for a possible quarter-final match if they receive a yellow card against France.
Several players in the French and Italian squad are teammates. Sagnol, Ribery vs Toni (Bayern Munich, Germany); Abidal, Thuram, Henry vs Zambrotta (FC Barcelona, Spain); Vieira vs Materazzi (Internazionale, Italy); Coupet, Boumsong, Clerc, Govou, Toulalan, Benzema vs Grosso (Olympique Lyon, France).